When labs require high throughput options and increased accuracy without the risk of human error or the need to retest for validity, an automated sequencing system is the best options.
The automated features of the Applied Biosystems sequencer models on the market offer a highly accurate, high throughput option to meet the needs of any testing or research facility. With different models and different sequencing capacity and options, this is a practical and important piece of equipment as a primary sequencer or to be used to add to the capacity of an existing sequencer.
Different Sequencing Options
The Applied Biosystems sequencer uses Sanger dideoxy sequencing that copies single strands of DNA through the addition of nucleotides to a chain from a template. This is completed by matching the paired base to the template as the extension extends.
The use of fluorescent dye labels in the process allows for the DNA extensions to be recorded based on the fluorescence. There are four different dyes used, one for each of the A, C, G and T extensions. The fluorescence is emitted as a light under different wavelengths, allowing for highly accurate reading in a single gel or capillary.
There is also the option to use an Applied Biosystems sequencer for cycle sequencing. This uses rounds of processing in a thermal cycler to extend the original template. Different kits, specifically the ABI Prism DNA kits are used with this method. Various kits are readily available to cover the full range of DNA sequencing requirements.
The various methods for sequencing using the Applied Biosystems instruments allow for flexibility as well as scalability in the lab. Most of the equipment has the option for upgrades to allow for increased sample analysis, increasing throughput without the need to replace existing equipment.