The CD4 is expressed on monocytes, lymphocytes, and granulocytes and is considered a cell surface glycoprotein. It has been implicated in cell migration, lymphocyte homing, tumor metastasis, and is a homing cell adhesion molecule, as well as a Phagocytic glycoprotein, Hermes-1, HUTCH-1, and ECM-III.
The CD44 antibody has a clone called SPM 521, and the isotype is the IgG2a. It has an undetermined epitope and a molecular weight of 80kDa.
It is designed for use with Western Blotting and Immunohistochemistry applications.
The IHC procedure includes using Formalin-fixed or paraffin-embedded tissues and deparaffinized slides, which can be achieved by using graded alcohols and xylene. You’ll find a pre-diluted formula that can be used with most IHC applications and will not require dilution. You can also use the concentrated format of the product and will need to dilute it using a ratio of 1:50 in an antibody diluent.
To retrieve the antigen, you should boil the tissue sections using a Citrate buffer with a pH of 6.0 for at least ten minutes, and then allow it to cool to room temperature for 20 minutes. You should also incubate it for 30 minutes while it stays at room temperature.
The positive control for IHC applications is the esophageal carcinoma with cellular localization occurring in the membrane.
For Western Blotting, you should use the concentrated version and dilute it using a ratio of 1:25. Because the dilution rates are different, it is not recommended to use the pre-diluted format. You will also want to incubate for one hour while at room temperature. The positive control for Western Blotting applications is the HeLa cell lysate, with cellular localization also occurring in the membrane.
The CD44 antibody is an excellent purchase for your lab and will help with a variety of research needs. Visit Spring Bioscience today to learn more.